analytical chem instrumentation question - main difference between a spectrofluorometer and spectrophotometer? I'm vaguely aware of the difference in emitted wavelengths but what are the structural differences between the two?
@nuts sorry to bother but would you mind taking a look at this question when you get a chance?
no worries if you're not sure, I'm going to ask my professors tomorrow anyway
I'm sorry I don't really have any clue. From online though it seems like the detectors for the spectroflurometer involve more sophisticated amplification schemes, presumably due to the less frequent occurrence of fluorescence.
Were you asking about the construction of the devices or the operation?
construction
yeah that'd be my best guess. From an optics standpoint, both utilize the same topology going in presumably because they need some kind of wavelength selector (done via some kind of dispersion mechanism, either a prism or a diffraction grating) for a monochromatic input, but the output stage i guess is where things can be different, and a cursory look online seems to suggest so... the interesting thing is most photodetectors aren't linear, especially the sensitive ones, so the construction of such devices is probably quite challenging.
alright thanks ~
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