Question

Martin is studying sucrase activity. He added sucrose solution + sucrase solution + buffer of pH 5.6 in two tubes. He incubated them at 350C and 1000C respectively. When he performed a confirmatory test, only the first test tube showed enzyme activity. How would you justify the inactivity of the enzyme in the second test tube?

Answer 1

thoughts? as a major hint, consider that the second test tube has a significantly higher temperature, and how that would affect the enzyme at a molecular level