Question

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Answer 1

PCR consists of three steps: denaturation, primer attachment and extension of the bound primer using Taq DNA polymerase.
In a cycle the DNA is heated op to 94°C which causes DNA strings will denature or separated. (1)
Cool down the DNA quickly to 60°C, whereby the primer binds to each template DNA strands. The two original DNA strings might regroup or compete with primer complementary binding sites. (2)
Finally heat up to 72°C, which Taq DNA polymerase extends the complementary DNA strands starting from the primer and the final finished copy of DNA. (3)
NOTE: Taq polymerase works most efficiently at 72°C, why it's important to hit exactly this temperature

Answer 2

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