Question

Hello! In PCR technique, what is amplified? The whole DNA molecule of the chromosome corresponded to the gene of interest or just the region corresponded to the gene itself? How is it determined?

Answer 1

Hello the meaning of amplification is nothing but making of multiple copies of DNA sequence (replicate)

PCR is capable of producing enormous amplification (i.e. identical copies) of a short DNA sequence from a single molecule of starter DNA.

It is used to amplify a specific DNA (target) sequence lying between known positions (flanks) on a double-stranded (ds) DNA molecule.

The amplification process is mediated by oligonucleotide primers that, typically, are 20-30 nucleotides long. The primers are single-stranded (ss) DNA that have sequences complementary to the flanking regions of the target sequence. Primers anneal to the flanking regions by complementary-base pairing (G=C and A=T) using hydrogen bonding.

The amplified product is known as an amplicon.

The gene of interest usually has to be amplified from genomic or vector DNA by PCR (polymerase chain reaction) before it can be cloned into an expression vector.

So, The whole DNA molecule of the chromosome corresponded to the gene of interest only