Hi, I am new here and actually to this field. I had a question and was wondering if you may have an answer?

I am doing an experiment at the lab. what I have done so far is: I did transform a plasmid DNA to E.coli. then I grow up 6 clones and extract the DNA from each one using Qiagen kit. I did run agarose gel electrophoresis to analyze the previous procedure (however, I did not have a good separation and never know how to summarize the result). then I did digest the DNA and run a gel (but I had the same problem before). after that I did express the protein form each clone.I need to do more work on these clone, but they got contaminated and do not know how can I get the same clones back out of this contaminated plates. Any idea?

Question

Hi, I am new here and actually to this field. I had a question and was wondering if you may have an answer?

I am doing an experiment at the lab. what I have done so far is: I did transform a plasmid DNA to E.coli. then I grow up 6 clones and extract the DNA from each one using Qiagen kit. I did run agarose gel electrophoresis to analyze the previous procedure (however, I did not have a good separation and never know how to summarize the result).  then I did digest the DNA and run a gel (but I had the same problem before). after that I did express the protein form each clone.I need to do more work on these clone, but they got contaminated and do not know how can I get the same clones back out of this contaminated plates. Any idea?

shrutipande9 · Answer

hi..try posting this ques in the biology section...i am sure people can help u out... http://openstudy.com/study#/groups/Biology