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While testing amylase activity, John uses a buffer of pH 6 and substrate starch. He incubates them at 104ºF (40ºC). All the tubes show glucose formation, except one, which had no starch solution. Why did this happen?
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Amylase is an enzyme that catalyses the breakdown of starch into sugars. Why do you think, if you do not add starch to the solution, no sugars will be formed? It's like you have 4 test tubes filled with water, you heat up the first three to 100ºC. why does the water in the fouth tube not boil?
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