Question

1) Use of plasmids and restriction enzymes in bacterial transformation and cloning.
2) Bacterial transformation lab procedure and expected results.
3) Use of electrophoresis in determining size if molecules.

Answer 1

Hi! What's the question? @alysku

Answer 2

@EclipsedStar can help with the process and/or how to do any one of these. Like how are plasmids and restriction enzymes used in bacterial transforming?
What are the Bacterial Transformation!ab procedures are and what are it's expected results.
how do gel electrophoresis used to determine molecule size?

Answer 3

Hey! @alysku !
I'll try to help you If I can...but i need your cooperation..Ok?

Answer 4

Alrighty @shreehari499

Answer 5

...Is this a lab? :o

Answer 6

Do you know the theory?

Answer 7

or should i tell you about what it is as well?

Answer 8

This a review for a test tomorrow @EclipsedStar "Gene to Protein study guide "

Answer 9

What's the theory?

Answer 10

Like...What plasmid is or what Restriction enzymes are...

Answer 11

the basics

Answer 12

A restriction enzyme is what is what keep the enzymes from expressing ?
And I'm not sure what a plasmid is.

Answer 13

No wait that's wrong

Answer 14

A plasmid is a self replication extra chromosomal DNA which has certain sites where we cut it with the help of restriction enzymes and add the expression the we need

Answer 15

OK then , I'll help you with the question you need and if you have any doubtts you are free to ask me..OK?

Answer 16

Yeah sure thing!

Answer 17

just a sec..I'll just go through my theory..

Answer 18

OK.'
Plasmids can be used as vectors to carry foreign DNA into a cell

Answer 19

An essential plansmid consists of,
AN Origin of replication (ori)
Selectable marker
Cloning sites

Answer 20

A restriction enzyme as told earllier, is used to cut the plasmid at specific location and helps in the inserting of the required foreign DNA into the plasmid

Answer 21

Ori is where synthesis occurs ?

Answer 22

So *cut* *insert specific DNA *

Answer 23

Exactly! Its the region in the plasmid where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells

Answer 24

YeAH...! 'CUT' using restriction enzymes and insert the foreign DNA...(Its easy to tell but very difficult to do it practically)

Answer 25

Transformation is a procedure through which a piece of DNA is introduced in a host bacterium

Answer 26

DNA means 'RECOMBINANT DNA'

Answer 27

I got it from another website but i think it'll be useful

Answer 28

Recombination DNA is the plasmid WITH the foreign DNA

Answer 29

The image makes a little easier to understand

Answer 30

Exactly! Sorry for being late

Answer 31

No problem :)

Answer 32

To tell you about electrophoresis I need to teach you about how to foreign DNA is isolated from its parent...

Answer 33

For that the following steps are used:
1) Isolation of the Genetic Material (DNA)
2) Cutting of DNA at Specific Locations (Thats Electrophoresis)
3) Amplification of Gene of Interest using PCR
4) Insertion of Recombinant DNA into the Host
Cell/Organism

Answer 34

You there? or is there any doubt?

Answer 35

I'm just trying to process the information

Answer 36

OK!

Answer 37

What do you mean by amplification of gene of interest ?

Answer 38

In simpler words: Isolate the required FOREIGN DNA, insert it into the plasmid you require and multiply the Recombinant plasmid and market it...

Answer 39

Amplification means MULTIPLYING or getting multiple copies of the gene of interest i.e recombinant DNA( we need more recombinant DNA to insert it into different plasmids) So in simpler words, more the no. of recombinant DNA more will be the Recombinanat plasmid

Answer 40

Oh okay I understand now

Answer 41

If you were given. A restriction map ( a plasmiid), how do you calculate how large the plasmid is.

Answer 42

sorry i do not have a good idea abt that

Answer 43

Most probably Electrophoresis but im not sure

Answer 44

Oh that's okay, I'll just ask my teacher real quick before the test starts tomorrow.

Answer 45

Ok and ALL the Best for you test! Do well!

Answer 46

Thanks for the help!

Answer 47

Anytime! And Bye!