To create bacteria that produce human insulin scientists insert the human gene of interest into the bacterial
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Inserting genetic material via plasmids is common when producing clones of bacteria for purposes such as insulin production.
The human insulin gene, which is located on the top of the short arm of chromosome 11 in human DNA, is cut from the DNA strand using restriction enzymes (genetics scissors). A plasmid (floating circular disks of DNA in bacteria) is extracted from a bacteria and cut open with another restriction enzyme, and the gene for human insulin is taken up by the plasmid. Another enzyme, ligase, is used to permanently seal the exposed nucleotides (ends of the DNA strands) together (like genetic glue). the plasmid is then put back into the bacterial cell, and the bacteria will then manufacture insulin. its offspring will also have the genetic data for human insulin.
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