Question

I'm running short on time and I need help understanding this problem.

Answer 1

1.70 x 10-5 M ML was found to generate a detector response of 426 mV at 532 nm. When a non-absorbing species was placed into the cuvette, the detector response measured was 765 mV at 532 nm. Calculate\[\epsilon \].

Answer 2

\(\epsilon\) is the dielectric constant?

Answer 3

Or molar absorptivity constant?

Answer 4

molar absorptivity

Answer 5

do I use the mV as power in vs power out?

Answer 6

mV is the reading. I could be mistaken, but it seems pretty simple. Basically, the second reading is the blank.

\(A_1-A_2=\epsilon*(M_1-M_2)\rightarrow \epsilon=\dfrac{A_1-A_2}{M_1-M_2}\)

Answer 7

that's just Beer's law, btw

Answer 8

M=mass? and A=absorbance...
I understand beer's law just not in this context.
I know nothing about the blank but the reading. I just don't get it

Answer 9

M is the molarity, and A absorbance.

The second solution is the blank because it contains no absorbing species.

This is analogous to finding the slope of a line using y=mx+b using 2 points.

Except here the two points are A and M

\(\sf A=\epsilon*l*M\)

Answer 10

except the two coordinates are A and M**, i meant

Answer 11

solution 1: \(A_1=\epsilon*l*M_1\)

solution 2: \(A_2=\epsilon*l*M_2\)

solution 1 - solution 2

\(A_1-A_2=(\epsilon*l*M_1)-(\epsilon*l*M_2)\)

Answer 12

i gotta get going, hope this makes more sense. Let me know if you still have doubts, ill check it out tmrw