Lets say we have a new disease. There is some protein 'x' that's causing the disease in humans. So how do we find this protein 'x'? What are the experiments we need to this protein?

Question

anonymous · Answer

well you would need to use a "process of elimination" type experiment that isolates the protein. You could then test for the dna sequencing then test that  against protein coding that you already knew of.

anonymous · Answer

Yes, I wanted to know how to do that process of elimination. 

I assumed that once u can get the bacterial/viral protein which is causing the disease, we can always get the DNA sequence using reverse translation and compare the gene sequence with the existing database we have and make it a potential drug target.

But I'm not sure how we actually extract the protein. Once if we can extract protein, we can probably do Mass Spectrometry to get its structure.

anonymous · Answer

euuhh

anonymous · Answer

well it would be comparative wouldn't it. Thats why you would be doing the dna sequencing in the first place. Your eliminating through the results.