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MIT OCW Biology
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Does an enzyme use a substrate, and cause a reaction to make a different molecule, that is termed the product. Such as in the substrate glucose, in the first step in glycolysis?
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Glycolysis comprises the 10 steps, which are catalysed by different enzymes. In the first step glucose is converted to glucose-6-phosphate by the enzyme:glucokinase or hexokinase. this enzyme is utilizing ATP and mg2+ ions.
To answer the first part of your question: yes. There can be a single substrate complex or a multiple ones. During their reaction with the enzyme on the active site, the subtrates within the complex creates a new chemical composition through the enzymatic mechamism (forming the product), but the enzyme itself doesn't change.
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