What is a limitation of using electron microscopes to view specimens?

You cannot view transparent specimens because of the microscope’s low resolution.

Electron beams are dangerous and can only be used sparingly.

You cannot view live specimens because the necessary preparation kills cells.

Resolution is weak and cannot make out details of very small specimens.

Question

What is a limitation of using electron microscopes to view specimens? 

 You cannot view transparent specimens because of the microscope’s low resolution.
  

 Electron beams are dangerous and can only be used sparingly. 

 You cannot view live specimens because the necessary preparation kills cells. 

 Resolution is weak and cannot make out details of very small specimens.

anonymous · Answer

Electron microscopes are used specifically for their high resolution and magnification power - so you should eliminate 1 and 4.

However, a notorious drawback to such high magnification is that the organism must undergo a procedure to 'prepare' it for viewing. Such procedures include applying a layer of heavy metal, freezing the organism, stabilizing it with a layer of proteins, and more...which are all lethal to the organism. So, only nonliving specimen can be observed.