Question

ok so I have a project that needs to be done but im out of town and no way of doing this, if someone can do the project for me you will be saving my life. You don't have to do any heavy writing, ill do that, just write down observations for me?

Answer 1

For this lab you will need: a blender, strainer, several small glass containers, water, salt, liquid detergent, isopropyl (rubbing alcohol), a DNA source (such as split peas, strawberries or liver), and an enzyme source (such as meat tenderizer or pineapple juice).
Step 1: First, you need to find something that contains DNA. Since DNA is the blueprint for life, everything living contains DNA. For this experiment, we like to use green split peas. But there are lots of other DNA sources too, such as:

Spinach

Chicken liver

Strawberries

Broccoli
Step 2: Put in a blender:

1/2 cup of split peas (100ml)

1/8 teaspoon of table salt (less than 1ml)

1 cup of cold water (200ml)
Blend on high for 15 seconds. The blender separates the pea cells from each other, so you now have a really thin pea-cell soup.
Step 3:

Pour your thin pea-cell soup through a strainer into another container (like a measuring cup).

Add 2 tablespoons liquid detergent (about 30ml) and swirl to mix.

Let the mixture sit for 5-10 minutes.

Pour the mixture into test tubes or other small glass containers, each about 1/3 full.
Add a pinch of enzymes to each test tube and stir gently. Be careful! If you stir too hard, you'll break up the DNA, making it harder to see.

Use meat tenderizer for enzymes. If you can't find tenderizer, try using pineapple juice or contact lens cleaning solution.
Step 5:

Tilt your test tube and slowly pour rubbing alcohol (70-95% isopropyl or ethyl alcohol) into the tube down the side so that it forms a layer on top of the pea mixture.

Pour until you have about the same amount of alcohol in the tube as pea mixture.

Alcohol is less dense than water, so it floats on top. Look for clumps of white stringy stuff where the water and alcohol layers meet.
DNA is a long, stringy molecule. The salt that you added in step one helps it stick together. So what you see are clumps of tangled DNA molecules! DNA normally stays dissolved in water, but when salty DNA comes in contact with alcohol it becomes undissolved. This is called precipitation. The physical force of the DNA clumping together as it precipitates pulls more strands along with it as it rises into the alcohol. You can use a wooden stick or a straw to collect the DNA. If you want to save your DNA, you can transfer it to a small container filled with alcohol.
Write a two-paragraph report on your DNA extraction. Be as honest as possible. Were there problems? Were you able to extract the DNA?

Answer 2

I would suggest explaining to your teacher that you're going to be out of town and that you need an extension.

Answer 3

I did but the problem is there are no extensions because this isn't like your regular highschool course, its 4 weeks and its a make or break you. Those are her words exactly, its fine if you can't do it ill find a way if nobody can help.

Answer 4

It's just... the whole point of this assignment seems to be to get you to see what will happen with your own eyes. Nobody can do that for you.

Answer 5

Well yeah I see what your saying but I also have to write a 3 page essay and this really plays into it I guess, Im willing to do it im not trying for ppl to do my work for me im just in a bad situation at the moment

Answer 6

Well... looking at the instructions, it doesn't look like it'll take long. Maybe take a short break from essay writing and give it a shot.

Answer 7

no see the problem is getting the materials to do this, im in a hotel room out of town and my budget is very limited its not like i can go out and do this, and in order to start my essay i need this done first. like i said its cool if you can't i just figured id give asking on here a shot

Answer 8

*go out and buy this