A researcher while working in vitro (outside cell) added helicase enzyme which resulted in the untwisting of the DNA helix. But this untwisting led to strain ahead of the replication fork. Which enzyme can be added to correct this problem? A. Nuclease B. Topoisomerase C. Telomerase

Question

anonymous · Answer

Topoisomerase

anonymous · Answer

topoisomerase can be added because it is responsible for converting a positive superhelicity DNA molecule to negative superhelicity molecule..... they are responsible for topological changes in the DNA (like unwound the DNA by breaking the phosphate backbone to remove the twist and sealing them)