Question

Outline how a true breeding black rose could be produced using the process of biotechnology (recombinant DNA), discuss the benefits and risk of breeding this black rose and its ethics of patenting this plant if it succeed.

Answer 1

Is there a specific difficulty you're having?
otherwise: how do you produce ANY GM plant?

Answer 2

my teacher said that the only plant that can naturally produce black colour allele is the Black Coral Pea plants and i think my teacher want us to outline how the black allele from Black Coral Pea plant can be transferred to a rose plant so it will produce a natural black colour too.. but i dont know how they can transfer it, they'll need a homozygous (AA or aa) black allele.. :( its so confusing..

Answer 3

Hmm (disclaimer: I haven't taken genetics, only plant bio).
If your textbook doesn't have a section on the process, you could try a plant bio book (if you have one). Wikipedia has an outline in the article on genetic engineering.

The jist of it is that the gene is taken from the source organism's genome (possibly using a protein sequencer to produce a synthetic gene). Otherwise the gene is cut from the genome using a restriction enzyme and spliced to a self-replicating bacterial plasmid (which has also been cut by the same enzyme). E. Coli might be used to take up and reproduce the gene many times over (so you have enough to work with). Finally the gene is again isolated and given to another bacterium (such as Agrobacterium tumefaciens) which will infect plant cells and inject their DNA into the genome of the infected plant's cells. Those cells are then cultured into full plants and bred traditionally to isolate the desired trait. (from Bidlack and Jansky, Intro. to Plant Biology p. 252-6)

My summary may not have enough depth for your class.

Answer 4

thanks for the help though :)

Answer 5

Btw how does we know which plasmid is suitable for the gene to combine with?