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Biology 19 Online
OpenStudy (anonymous):

Why is the fact that DNA has a negative charge so important in the gel electrophoresis process?

OpenStudy (anonymous):

Since the DNA has a negative charge, when the current runs through the gel, it allows the DNA to move towards the positive end of the chamber. lol got the answer

OpenStudy (koikkara):

yeah,....i too was thinkin, thanks for tip....well, DNA has a negative electric charge, and is attracted to the positive electrode. The gel resists the DNA as it moves; smaller pieces have an easier time moving through the pores in the gel and so travel further. Given a known gel preparation and electrical application, the length of a segment can be very precisely determined by the distance that it travels. It can then be cut from the gel using a scalpel.

OpenStudy (anonymous):

here a medal anyway

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