While testing amylase activity, John uses a buffer of pH 6 and substrate starch. He incubates them at 104ºF (40ºC). All the tubes show glucose formation, except one, which had no starch solution. Why did this happen?

Question

thomaster · Answer

Amylase is an enzyme that catalyses the breakdown of starch into sugars.
Why do you think, if you do not add starch to the solution, no sugars will be formed?

thomaster · Answer

It's like you have 4 test tubes filled with water, you heat up the first three to 100ºC. why does the water in the fouth tube not boil?

anonymous · Answer

so whats the answer

anonymous · Answer

A) the optimum pH for enzyme amylase is not pH 6
B) the optimum temputure  for the enzyme amylase is not 104. F (40.)
C) the substrate for amylase, starch, is not present

anonymous · Answer

its not b.

anonymous · Answer

so whats the answer

thomaster · Answer

You guys only seem to care about the answer. If you read what I wrote a year ago and think a little, how could not be able to solve this problem...

Starch is one of the two ingredients for this reaction along with amylase. Those 2 combined will create sugar. If you do not add starch in the last test tube, obviously there won't be any sugar right? because you didn't add one of the 2 necessary ingredients.

anonymous · Answer

IF PLATO C