Question

why misincorporated nucleotide do not lead to mutant progeny during dna synthesis in e coli

Answer 1

I don't know what specifically you are asking for, but as far as I can understand a mutant is one which shows a phenotype, so first of all if that nucleotide doesn't result into any phenotypes you won't be calling it a mutant. Other than that, to answer the specific question, a misincorporated nucleotide can lead to no effects in translation because of the wobbling effect. This is because of the multiple number of codons that a single amino acid has. Hence, if that misincorporation is leading to a change in base at the third place of a codon, it most likely won't create a mutation. This is termed as a silent mutation, because it doesn't result into any noticeable phenotypes. I hope this helps! Let me know if you have any more specific questions about it. :)