1. How does the process of gel electrophoresis separate DNA fragments?

2. What is the purpose of the agarose gel?

3. What is the purpose of adding blue “tracking” dye to the DNA samples?

4. Explain why DNA has an overall negative charge.

5. Why is the fact that DNA has a negative charge so important in the gel
electrophoresis process?

6. Explain how an agarose gel can separate DNA fragments of different lengths.

7. What is the purpose of ethidium bromide?

8. Why is a marker used when running the fragments through the gel?

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Question

1. How does the process of gel electrophoresis separate DNA fragments?




2. What is the purpose of the agarose gel?




3. What is the purpose of adding blue “tracking” dye to the DNA samples?




4. Explain why DNA has an overall negative charge.




5. Why is the fact that DNA has a negative charge so important in the gel
electrophoresis process?




6. Explain how an agarose gel can separate DNA fragments of different lengths.




7. What is the purpose of ethidium bromide?



8. Why is a marker used when running the fragments through the gel?




9

aaronq · Answer

lol  translation: "do my assignment!!!"

anonymous · Answer

LOL @aaronq

some relevant info though:
•Current is applied in electrophoresis
•Charged and polar particles are effected by this
•DNA has charge 
•Size of DNA matters for speed of movement
•Put in some effort!

Sorry, just had to go there. Please keep asking questions though!