Question

What does it mean to have a protein serve as a tag for the isolation of recombinant proteins?

Answer 1

This has to do with fusion proteins. I can't fully understand what's in my textbook.

Answer 2

yep i also think it is fusion proteins...i had read about one....fusing a protein of interest with gfp(green fluoroscence protein) so that while isolating we can actually locate our protein by making it visible under uv. since gfp gives green colour under uv

Answer 3

hope this helped...btw what is given in ur book?

Answer 4

my text says it's using recombinant DNA methods to insert the gene for a protein of interest into a plasmid containing a gene for a well-known protein that serves as a "tag". the tag protein then allows for the isolation and purification of the recombinant protein as a fusion protein.

Answer 5

it's for an introductory Biotechnology class

Answer 6

ahh...now i get it...see lets take an eg...we want to isolate gene which codes for beta galactosidase. and we take a plasmid which has a gene which codes for ampicillin resistance

Answer 7

now to make this recombinant dna we fuse the gene of beta galactosidase and plasmid and then let the bacteria take up this plasmid.

Answer 8

after a day maybe we plate these bacteria on a medium with ampicillin. so only those bacteria having the mapicillin resistant gene would grow on this medium...right?

Answer 9

yeah

Answer 10

so then those bacteria which grow would also have our gene of interest..so we have used the tag of ampicillin resistant gene to isolate our gene of interest

Answer 11

Okay...so the tag is a marker then? An identifier?

Answer 12

yes..absolutely right..:)

Answer 13

:) thanks!

Answer 14

happy 2 help:D