Question

Please Help with Replication, Transcription, and Translation:-D

Original DNA Strand: TAC GAC CCG GCC AGG TCA

1. Replication: What will the complementary base sequence be?

2. Transcription: What will the mRNA code be?

3. Translation: What amino acid sequence will be craeted?

Answer 1

Dna replication works on the principle of base complementarity which is
A pairs with T
G pairs with C

mRNA synthesis / transcription also works on the principle of base complementarity
A pairs with U
G pairs with C

this would help you in answering your first 2 questions.

Answer 2

i will help you with last one once you finish these two

Answer 3

so in the second one, will the T in the DNA strand stay T?

Answer 4

yes it will remain as T , anyways there wont be any changes in DNA it is the RNA that is being synthesized. Dna only acts as a template.

Answer 5

OK, I have the first 2 done, how do u do the last one (translation)? :-D

Answer 6

are you sure you've got hem right ? because your third answer depends on your second answer.

Answer 7

I got: TUG CUG GGC CGG UCC TGU for transcription

Answer 8

sorry you have T in your template DNA , write the complementary base of T in the RNA.

Answer 9

HUH? so do an A instead of T?

Answer 10

yup , how can a T in dna pair with a T in RNA. are they complementary to each other.?

Answer 11

so it would be AUG CUG GGC CGG UCC AGU?

Answer 12

bingo !! that's right .

Answer 13

Thanku so much!!!

Answer 14

now go to the codon table to write the amino acids sequence . I will help you with the writing the first amino acid and expect you to write the rest. We will use the circular codon table it is easier.

Now the first codon is AUG.
1. go to the A quadrant of the circle (as A is the first nucleotide)
2. then go to the U subdivision of the A quadrant ( since U is the second nucleotide)
3. then go to the G subdivision of U in the A quadrant ( since U is the third nucleotide )
You get your first amino acid that is glycine
Choose the quadrant depending on the first nucleotide.

Take your time and write the remaining amino acid sequence.

Answer 15

Im xtrmly sorry the first amino acid is METHIONINE and not glycine , sorry for the confusion.

Answer 16

once u write the amino acid sequence do show me.

Answer 17

Ok, I got: Methionine, Leucine, Glycine, Arginine, Serine, and Serine. idk if I spelled these right:-D

Answer 18

absolutely right !!! congratulations.

Answer 19

Thanks for all ur help!

Answer 20

you are welcome and do ask if you have any other doubts. We are always there to help you.