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Biology 15 Online
OpenStudy (playa):

what property of dna causes it to migrate tot he positive pole of the electrophoresis apparatus? a.the positive charge of the dna b.the negative charge of the dna c.sugars found in dna d.nitrogenous bases in dna

OpenStudy (playa):

so i know dna is negative but since its migrating to positive pole i dont know if it like changes to positive

OpenStudy (shreehari499):

OK..so if u know that DNA is negative....so whats the doubt?

OpenStudy (shreehari499):

the negative DNA gets attracted to the positive pole of the electrophoresis apparatus and gets arranged in their ascending order according to their size..:)

OpenStudy (playa):

because it can either be the negative like you said and it can also be the nitrogenous bases

OpenStudy (shreehari499):

hmm..u r rite...the nitrogenous bases are negatively charged and they in turn are responsible to the overall negative charge of DNA.......BUT ITs NOT A PROPERTY OF DNA's NEGATIVE CHARGE..:)

OpenStudy (playa):

so im thinking it could be nitrognous then.....

OpenStudy (playa):

thanks

OpenStudy (shreehari499):

What..u got the answer?

OpenStudy (playa):

like i told you dna is originally negative and the question says property of dna causes it to migrate to the positive pole of the electrophoresis apparatus so since its like the opposite it narrows it down to b and d but since it not really likely to be b then its d

OpenStudy (shreehari499):

lol...thats kinda confusing! So at last D!

OpenStudy (shreehari499):

@alphadxg ..what do you think?

OpenStudy (playa):

yea its confusing

OpenStudy (alphadxg):

Yea, It's D, because of the Negative charge in the DNA backbone.

OpenStudy (playa):

i thought so thanks

OpenStudy (shreehari499):

why cant it be b? @alphadxg ..both are the same!

OpenStudy (alphadxg):

The backbone is the reason why it's negative in the first place @shreehari499

OpenStudy (shreehari499):

:)

OpenStudy (shreehari499):

yeah..option b is also rite...but to be precise..option d is correct!

OpenStudy (playa):

yes

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