Question

what is the mechanism explanation behind in every cell staining?

Answer 1

https://in.answers.yahoo.com/question/index?qid=20090227193635AA3jQ70

Answer 2

does this mean you already got your answer?

Answer 3

well basically, you have a dye (like DAPI, for instance) that interacts SPECIFICALLY with a molecular motif of the cell (in this case, AT-rich DNA sequences). in many cases, the dye changes colour in the course of the cellular staining reaction.

for classical stainings, you will want to kill and fixate the cells first. thus you ensure that nothing is moving inside when you apply the dye. you will then proceed to apply the staining solution, incubate, and wash away excess solution.

Answer 4

The reason that staining is used to stain cells is because it allows different parts of the cell to adhere to the different colors of stain. Gram stain has 4 colors and differential stains have 3, The deep purple part of the stain will show the inner part of the cell such as in blood cells if it is a monocyte or a segmented neutrophil, Without staining the small details that are needed for particular defects in the cells cannot be seen or will not be as clear as with the stain.

Answer 5

Each color has a purpose and is placed for x amount of time in each color on the microscope slide then transferred to the next and so on, at the end the slide is air dried or blotted with a special paper that will not cause the stain to be removed ope tis helps

Answer 6

Yes @jeeppgirl explained the mechanism well !
As a side note:
Why staining is done ?
It's because most of the cells/tisssues would not reflect light rays properly due to there transparent nature. Staining allows the coloured stain particles adhere with the specimen and thus allow them to reflect light which is then seen our eyes via an optical instrument.

Answer 7

@theyaana @jeeppgirl

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Answer 8

Thank you You all really make this a great experience, I will be as helpful as I can to anyone who needs it. Learning is great and a lot of fun once you really get going and feel like someone is there for you

Answer 9

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Wish u all the best !
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Answer 10

Thank you everyone,, This is an absolutely fantastic site and I am thrilled to help whenever and whoever needs it. I do give explanations also otherwise you wont learn why the answer is correct and have full understanding

Answer 11

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Answer 12

well i missed out.....

Answer 13

jks, its fine

Answer 14

@jeeppgirl @theyaana
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Answer 15

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Answer 16

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Answer 17

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Answer 18

ohmyGod! im so sorry i missed this :( anyway @Abhisar @jeeppgirl, THANK YOU SO MUCH! :)))) thank your for the help, big help much ^_^

Answer 19

unfortunately, i already pass my homework yesterday :/ anyway thank you for the idea ^_^ thank you so much everyone esp. @jeeppgirl and @abhisar