Give the amino acid sequence of the polypeptide produced by this gene. Be sure to enter the sequence N- to -C from left to right. Do not include the "N-" or "-C". Please use the 3-letter amino acid abbreviations (for example, "Ala-Gly-Ser"). We will ignore spaces and dashes when we grade your answer.
This is the gene in the question :)
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please do not expect direct answers i like you to atleast try for it
Can you share your thoughts my friend...!!
Oh believe me I've tried :P I'd love to work with someone on it if possible but I genuinely dont understand the question :(
can you say what is the N- in the question
Honestly no, Ive been using a chart I found to show the amino acids associated with each set of three bases. Ive been starting from where the blue ends on the top strand (11th Base).
it might be T not N according to me @JFlaus
You mean that Im beginning in the wrong place?
yeah obviously... i mean it
can you tell one thing..
So N is a representative of the beginning of the first codon? Meaning that I was starting at the eleventh base when I should have been starting somewhere else right :) Any idea where I should start then? :) Thanks for the help :D
what do you think the blue one is..
The blue section is the promoter I think?
there is no amino acid starting with letter N nor its Nitrogen [it cannot be nitrogen but also if we think about it]
Oh I know that, N doesnt have the same meaning as A,T,G,C (+U in RNA). So when transcription begins after the promoter you have the bases being transcribed until the stop codon? So N = Promoter and C = Stop Codon?
yeah i know that it was just to make you feel better and think about the answer in this way....
So I'm right in saying that above? I've just been trying to work it out here with your help :)
okay and sorry i responded late was busy somewhere else
Thats absolutely fine, You have other things to do aswell :) So I've got an answer but Im still not sure if its right. Starting to transcribe where the asterix is I got Trp-Thr-Thr-Gly-Asn then a stop codon. Is that right to say that the amino acid sequence of the produced polypeptide is Trp-Thr-Thr-Gly-Asn then? :P
then when a stop codon is there the protein chain breaks and then again starts next to the stop codn the protein chain goes on synthesises right..??
Yes, but after the stop codon then its synthesising a different polypeptide isnt it? And the question only asks for one polypeptide sequence :)
ya leave about the question ... if u try further you may get it practiced
and more over the question asked only one polypeptide chain so only gave one only promoter right..??
So the first sequence is definately Trp-Thr-Thr-Gly-Asn and theres only one promoter in the question so I can only give one sequence. To give another sequence I would need another promoter to re-start transcription. I understood transcription basics and now thanks to your help I think I definatley have a better grasp :D Thank you very much :)
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give the sequence to them who ever is concerned if its correct its good or come back here we will try it again okay...???
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