Question

friends....IF THE SPECIMENS WE WANT TO OBSERVE ARE NOT TRANSPARENT THAN HOW TO SEE THEM THROUGH MICROSCOPE?

Answer 1

what features of the specimen do you want to see..?

Answer 2

u there..!

Answer 3

hi @rusbhara ..let me know about the type of specimen you are looking at..because microscopic techniques depend on what actually you want to observe

Answer 4

@biocrystal...i m studying microbiology now first year...so our sir asked us question that if a specimen is not transparent then ow to see it through microscope? now the specimen might be the colonies of different kinds of bacteria..so help me here

Answer 5

a phase contrast microscope helps to look at such things..try reading more about phase contrast microscopy

Answer 6

there will be some media ' s like saffranin, crystal blue that give colour to the specified part of the bacteria which helps us to learn them

Answer 7

ohh is it so @BIOCRYSTAL i didnt knew about that type of microscope [never heard before]

Answer 8

ok phase contrast microscopy ...ok i read about those in little..so that might help me. thanxxx...BIOCRYSTAL AND deepika.comet

Answer 9

but being not transparent itself is the main thing one needs for better study especially in microbiology..most of the times bacteria being transparent causes a great problem to observe under microscope..that is why we use stains to create a phase difference and make it easily observable with human eye under microscope..

Answer 10

ahaaaaan so i was a bit correct right..?

Answer 11

that's right @deepika.comet. but the question here is not about transparent things..
so transluscent things are already good enough to be seen under a phase contrast microscopy without any staining

Answer 12

ohh k thanx for thing you shared me @BIOCRYSTAL