medal included!!!
While testing amylase activity, John uses a buffer of pH 6 and substrate starch. He incubates them at 104ºF (40ºC). All the tubes show glucose formation, except one, which had no starch solution. Why did this happen?
A) the optimum pH for enzyme amylase is not pH 6
B) the optimum temputure for the enzyme amylase is not 104. F (40.)
C) the substrate for amylase, starch, is not present

Question

medal included!!!
While testing amylase activity, John uses a buffer of pH 6 and substrate starch. He incubates them at 104ºF (40ºC). All the tubes show glucose formation, except one, which had no starch solution. Why did this happen?
A) the optimum pH for enzyme amylase is not pH 6
B) the optimum temputure  for the enzyme amylase is not 104. F (40.)
C) the substrate for amylase, starch, is not present

abb0t · Answer

First, you need to know what amylase does: it catalyses the hydrolysis of starch into sugars. 
And as you may already know, enzymes have an optimum pH function, which is the point where the enzyme is most active. If it is too low (or too high) it may mess up the function of the enzyme or lost its function completely.