Question

Hey, I have another question, could you please @BioEpic have a look (I will post a picture). If you could help me with a i), I would try to do ii) and iii) and you could have a look at them?

Answer 1

@Abhisar hey,could you please have a look?

Answer 2

this is a site to study and cheat;)

Answer 3

it is not cheating?

Answer 4

not cheat sorry

Answer 5

Tell me about one said well I needed help on something and she said that i'm cheating when i'm not

Answer 6

and she also said she will susspend me too

Answer 7

@zepdrix maybe you can have a look?

Answer 8

hey @aaronq , any ideas?

Answer 9

What do these scores mean?

Answer 10

I don't know:/ maybe recombination frequency?

Answer 11

I dont think recombination frequencies apply here because this isn't meiosis, it's transduction.

I think these scores just mean the percent of the different types of phages that accepted the genes (dmc* in exp 1, etc.).

Do you know how that correlates to distance? What have you learnt about this in your course?

Answer 12

I really don't understand much about it:/

I know that distance and co-transduction is realated and the greater the meaning of co-transduction is, the closer two genes are to each other

Answer 13

ok, I found an example

Answer 14

Oh okay, so it's similar to recombination frequency in that sense.

I'm really not sure how to interpret the data, i'll around online for examples and comeback if I find anyhting

Answer 15

• Cotransduction of gal with bio can be studied by plating infected cells on medium lacking biotin
– Both gal+ bio+ and gal¯ bio+ cells will grow
• Then test for the gal marker leu gal bio 100 kb gal bio bio
• 42% are gal+ bio+ and 58% are gal- bio+
– The frequency of
cotransduction of gal and bio is
42% or 0.42


So according to this, dcm and fla frequency of cotransduction is 0,03 or 3%?

Answer 16

any ideas?

Answer 17

oh I saw you commented now, no worries: ))

Answer 18

following that logic, i think the distances between them are

fla and dcm 48%

dcm and his 13% though in experiment III, it was 10%

fla and his 0%

Answer 19

could you explain how you get these numbers?

Answer 20

well look at the chart, in experiment I, dcm* was tested for

fla- his- 36% only dcm* was transducted
fla* his- 48% both fla* and dcm* were transducted (similar to recombination)
fla- his* 13% both his* and dcm* were transducted

Answer 21

I understand like nothing :/ nevermind, it is too hard for me, thanks for your help:)

Answer 22

I will read some more and then try to do it again

Answer 23

no problem! it's also possible that i'm doing it wrong. If you still havent found your answer tag me and i'll pull out my genetics book and look through it.

Answer 24

ok, thanks, cheers!