Question

Hello, just want to ask if there any diferences between Gram - and Gram + in term of rate of decolorization? (Gram staining exp) i really appreciate answer from all of you

Answer 1

@syay43 It is not about the rate really. The reason the stain works is because of the differences in the thickness of the cell wall. Once the gram+ bacterium are stained, the stain cannot be easily removed with the chemical that does the destaining. Gram- bacterium are easily destained.

Answer 2

thnks a lot @mrdoldum :)

Answer 3

@syay43 This diagnostic can be difficult to do well. Technically the counter stain for gram- is not necessary. If the stain can be removed it is gram-. Another issue is that excessive destaining can damage the cell walls enough to ruin the results. Finally, some bacteria, like acid-fast bacteria, have a waxy coating that can confuse the reading of a gram stain.

Answer 4

oh..it ok actually our lab assistance already prepared specific bacteria.. hehe thnks a lot @mrdoldum you really a big help to me. about acid fast bacteria.. i use Mycobacterium phlei.

Answer 5

@syay43 Yeah, when I was a teacher's assistant for Microbiology Mycobaacterium really confused students on the gram stains.

Answer 6

wow that awesome..@mrdoldum you was a teacher assistant for microbiology at where?

Answer 7

@syay43 I prefer to not to give out such information due to the amount of geographical knowledge that would provide. However, I was a TA for 7 lab sections of Microbiology when I did my BS in Biology. I am currently in the last semester of my PhD in a molecular biology field, and teach intro bio labs at this moment.

Answer 8

owh ok i understand..forgive me to ask that question.What can i say is you such an inspiring person @mrdoldum