Question

I'm looking for research literature regarding antibodies specifically against Eimeria parasites. Preferably polyclonal since I'm trying to link the parasites to immunomagnetic beads in order to isolate them from poultry stool samples.
Anyone knows or read something about this?

Answer 1

No idea really, but i searched and found this, it seems to be a methods paper & its the earliest i could find
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858809/

Answer 2

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4135734/
http://www.ncbi.nlm.nih.gov/pubmed/24687282 this one seems to relate your topic, but you gotta buy this one

Answer 3

Thanks for looking!
First link is about identification with pcr, I need to isolate parasites from the samples before I identify them, that's where I'm stuck.

Second article looks more promising but they use antibodies against proteins in the cytoplasm, I need polyclonal antibodies that bind to surface proteins in order to link them to immunomagnetic beats.

Third article is where I got the idea of magnetic separation from ;)
But they use it for cryptosporidium, I want to know if there are known antibodies against Eimeria specifically.

Anyways thanks for the help :)

Answer 4

@Somy One question, where did you get that amazing anime pic? I love it<333

Answer 5

http://iai.asm.org/content/58/2/557.short @thomaster ?

Answer 6

http://naldc.nal.usda.gov/download/16235/PDF
http://www.google.com/patents/EP0135712A2?cl=en
http://iai.asm.org/content/80/5/1909.full

Answer 7

all these 4 are antibody related

Answer 8

@Crystalinq oh i love these arts, i have a lot of them + its not from anime lol

Answer 9

omg I want oneee<3

Answer 10

you can try answer . com on googl

Answer 11

I've done immunoprecipitation, which is similar to what you're talking about, thomato.
Using antibodies to separate or isolate a protein. I'm currently dong this.

Answer 12

I'm not isolating proteins, but unicellular parasite oocysts. Just need to find a way to bind them to magnetic beads so I can isolate these cysts from stool samples.

Answer 13

i searched in endnote, i typed 'immunomagnetic separation of parasite from stool' and selected the most recent ones
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2880624/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256281/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC262248/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC270532/
these ones caught my sight
specially one of them also deals with cysts (read abstracts)

Answer 14

didnt find anything specifically about parasite you want to separate, but just the technique

Answer 15

I know enough about the technique, just need to know about this specific parasite :p
But thanks for looking.

Answer 16

You are welcome though sadly couldnt find what u needed #_#

Answer 17

Yes I searched extensively and I think there is no research on this one yet.
Too bad, guess I'll have to find it out myself then.

Answer 18

good luck with it!! @thomaster
i'll ask if someone here in my uni deals with parasites