Question

Two important steps involved in genetic engineering are cutting the desired gene from its genome, then cutting the vector genome and pasting the gene into it.
How is this carried out?
-Using the same restriction enzyme for cutting the desired gene and the vector and using ligase for pasting the gene into the vector
-Using two different restriction enzymes for cutting the desired gene and the vector and polymerase for pasting the gene into the vector
-Using the same ligase for cutting the desired gene and the vector and a restriction enzyme for pasting the gene into the vector

Answer 1

Hey @lexielou WELCOME TO OPEN STDY !

Answer 2

thanks!! @Rushwr

Answer 3

So what do u think the answer is ?

Answer 4

im not sure honestly @Rushwr

Answer 5

Okai ,
1) Restriction enzymes are used to cut DNA into smaller fragments.
2) Ligase enzyme is used to bind DNA fragments with blunt ends.
So what do u think the answer is ?

Answer 6

the second one ?

Answer 7

oh wrong i forgot to mention about the polymerase enzyme, polymerase enzyme is used for proofreading” the strand so that misplaced base pairs can be corrected.

Answer 8

SO the answer is going to be A
B is wrong because we dnt use polymerase enzyme for pasting
C is wrong because Ligase enzyme is not used for cutting but for pasting

Answer 9

I hope u got it :)

Answer 10

i was gonna say that after reading it again! thank you so much, I definitely have it!

Answer 11

hahahaah no problem :)