Question

What do you need to do to determine if the genes on the plasmid are expressed?

Answer 1

There are no answer choices as this question is a free response question.

Answer 2

do you know anything about this topic?

Answer 3

hm im not sure

Answer 4

like insulin production using plasmids
have u ever heard of it?

Answer 5

i can tell you the process and how to test if gene is getting expressed but i need to know how much u know about this topic

Answer 6

I know about ho genetic engineering change the plasmid by using restriction enzymes to cut the donor gene out and make cut so the sticky ends can attach together.

Answer 7

Alright cool

Answer 8

So one of the ways to check if your plasmid is working or not

Answer 9

when you cut a plasmid and insert your gene of interest, you need to add one more gene sequence that will be beneficial for a bacteria

Answer 10

Like antibiotic resistance?

Answer 11

for example a gene that would give a bacteria resistance to an antibiotic such as penicilin

Answer 12

yes, so once you put that sequence and get the plasmid into the bacteria you need to culture it

Answer 13

That's reproduce it right?

Answer 14

and your agar has to include penicilin or whatever antibiotic towards which the plasmid has gene that can resist to it

Answer 15

that was you bacteria will have to replicate YOUR plasmid and get the resistance

Answer 16

you will see it in your culture plate

Answer 17

The way you explained this helped me so much! Thank you!

Answer 18

if your bacteria is able to grow in your culture means that you succeeded

Answer 19

or the other way would be adding a sequence from which you can get fluorescence emission

Answer 20

that way you can actually observe it happening

Answer 21

no problem :)