Question

Solving for Km:

I understand how I find Vmax, but I am having trouble solving for Kmax.....

Answer 1

I was thinking that slope = Km / Vmax
Km = slope x Vmax
Km = 7 x 10 ^-8 * (1/0.0071)
Km = 9.86 x 10^-6

Answer 2

but I know that - 1 / Km so is Km actually -1/9.86 x 10^-6 = - 1.0 x 10^-5

Answer 3

Hey @Summersnow8
You are correct in your calculation of \(K_{M}\) through the use of the slope in your Lineweaver-Burk plot.

The \(\frac{-1}{K_M}\) is equal to the value of the intercept with the x-axis and should equal the same.

Answer 4

I have done so much enzyme kinetics so if anything please just ask ahead. :)