Solving for Km:

I understand how I find Vmax, but I am having trouble solving for Kmax.....

Question

Solving for Km: 

I understand how I find Vmax, but I am having trouble solving for Kmax.....

summersnow8 · Answer

I was thinking that slope = Km / Vmax
Km = slope x Vmax 
Km = 7 x 10 ^-8 * (1/0.0071) 
Km = 9.86 x 10^-6

summersnow8 · Answer

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summersnow8 · Answer

but I know that - 1 / Km so is Km actually -1/9.86 x 10^-6 = - 1.0 x 10^-5

frostbite · Answer

Hey @Summersnow8
You are correct in your calculation of $K_{M}$ through the use of the slope in your Lineweaver-Burk plot.

The $\frac{-1}{K_M}$ is equal to the value of the intercept with the x-axis and should equal the same.

frostbite · Answer

I have done so much enzyme kinetics so if anything please just ask ahead. :)