I have a lab math question that's related to MolCell Bio but I don't know if this is the right place to ask. I'm going to give it a shot -
"You have a tube of primer and the label has fallen off, but you still have the sequence of the primer ordered [N=25]. You decide to make a spectrophotometer reading to determine the absorbance. A 1:100 dilution yields an A260 - 0.050. How would you prepare a 10 micro M stock solution of that primer for use in a PCR reaction?
Given: 1 A260 unit of ssDNA is equivalent to concentration of 33 micro g/mL.
Any help? =]

Question

I have a lab math question that's related to MolCell Bio but I don't know if this is the right place to ask. I'm going to give it a shot - 
"You have a tube of primer and the label has fallen off, but you still have the sequence of the primer ordered [N=25]. You decide to make a spectrophotometer reading to determine the absorbance. A 1:100 dilution yields an A260 - 0.050. How would you prepare a 10 micro M stock solution of that primer for use in a PCR reaction?
Given: 1 A260 unit of ssDNA is equivalent to concentration of 33 micro g/mL.
Any help?  =]

anonymous · Answer

Also given conversion factor for ssDNA to convert micro g to pico mol: 
10^6pg/1 micro g x pmol/330 pg x 1/N = pmol

anonymous · Answer

I guess you already know the right answer, let me try and tell me if is correct,
concentration of primer in the actual sample is 0.050x33micr g/mlx100=165microg/ml.
then prepare picomolar solution by further dilution according to the sequence