Question

how is the transcription terminated in eukaryotes? does it occur before splicing and addition of cap and poly a tail?

Answer 1

Transcription takes place in nucleus while addition of cap and tail takes place in cytoplasm ..
So transcription ends before addition of cap and tail..

Answer 2

ok thnx:) but what is the exact procedure?

Answer 3

Transcription is a process in which RNA is synthesized from DNA bY RNA polymerase . First of all some part of DNA uncoils There is a special sequence of nitrogenous bases on DNA called promoter , RNA polymerases attaches to promoter and moves . An RNA polymerase moves A single RNA strand is synthesized (complementry to DNA strand) There is another sequence of nitrogenous bases on DNA called Stopping Signal Or Stop codan , RNA synthesis completes here ...This whole process takes place in nucleus , Now , RNA comes in cytoplasm ..
As DNA is transcribed into RNA it needs to be edited to remove non-coding regions, or introns This editing process is called splicing, which involves removing the introns leaving only the , protein-coding regions, called exons.
RNA splicing starts with helper proteins at the intron/exon borders. These proteins guide spliceosome to form a splicing machine.The spliceosome then brings the exons on either side of the intron very close together, ready to be cut. One end of the intron is cut and folded back on itself to join and form a loop. The spliceosome then cuts the RNA to release the loop and join the two exons together. The edited RNA and intron are released and the spliceosome disassembles...

Answer 4

ty:)

Answer 5

got it @shrutipande9 ?

Answer 6

pretty much yes. actually when i was reading prokaryotic transcription it mentioned that there are 2 types of terminations rho dependent and rho independent. but likewise in eukaryotes i could not find anything.

Answer 7

Opss.. i dont knw anything about rho dependent and rho independent...

Answer 8

no probs....thanks:)

Answer 9

u'r wlcm c:

Answer 10

the addition of the 7-methylguanosine cap occurs in the nucleus (in the nucleoplasm) soon after transcription started, not in the cytoplasm (it needs it to exit through the nuclear pore). Poly-A tails are also added in the nucleus. As far as i know, how transcription ends in eukaryotes hasn't been determined.

Answer 11

Ty@ aaronq