Question

Report the location of sites in reference to Bgl II end

Answer 1

is this about restriction enzymes?

Answer 2

I'm basically creating a restriction map of a cDNA insert

Complete digestion of insert:
Hae II: \(\sf \color{blue}{0.2, ~0.4~, 0.6~,0.7~,0.9~, 3.0~, 0.7}\)
Bgl II: \(\sf \color{red}{0.5~,6.0}\)
Sal I: \(\sf \color{green}{2.4,~4.1}\)

Bgl II + Sal I: \(\sf \color{orange}{0.5,~1.9~,4.1}\)
@nincompoop

Answer 3

UGH, got cut off, but the bottom is Sal I,
i just don't know how to read these, how do I know which one is which cut on the Bgl II + Sal I???

Answer 4

dude, all of a sudden you're doing more complicated bio than I am

Answer 5

is this base off bacteriophage?

Answer 6

I suppose a plasmid, but I am working with a linear one. It makes it less confusing for me.

Answer 7

let me grab my genetics book
it looks familiar but I don't know if I even learned this

Answer 8

oh I think you just aligned it linearly

Answer 9

Yeah.

Answer 10

ok, so idk if this is heading int he right direction, but I am thinking that since there is a split in the double digest, and theres no longer a cut at 2.4, that the enzyme cut in between the 0.5 and 1.9! But which enzyme?!?!?! LOL

Answer 11

What do you think?

Answer 12

who gave you the numbers on Bgll?

Answer 13

those are just the hypothetical numbers assigned to keep the math relatively simple.

Answer 14

BGL II right? not BgL l

Answer 15

BgL II

Answer 16

If i have a double digest between
Bgl II + Cla I
where
Bgl I cuts are at: 0.5, and 6.0
and
Cla I cuts are at: 1.0 and 5.5


Bgl II + Cla I displayed bands at: \(\sf \color{red}{0.5,~1.0,~5.0}\)

Answer 17

I am so confused right now. lol

Answer 18

I am so overwhelmed rn

Answer 19

this is the kind of I just know

Answer 20

hmm...that helps a little, but the wording is so confusing. more confusing than it needs be.

Answer 21

I'm off 2 bed, thnx 4 teh help.

@aaronq any input?

Answer 22

On the Bgl II + Sal I, it's best to compare it to the Sal I only map.
As you see, on the Sal I map, you have a cut site at 2.4, which produces two pieces (2.4 and 4.1).

When combining the two, you have a cut site at 0.5 and 4.1...

Answer 23

Think about what the 0.5 and 1.9 piece add up to
So that means that the 2.4 piece that would normally be produced by Sal I has now been split into a 0.5 piece and a 1.9 piece by the Bgl II enzyme.

Answer 24

Is this related to micro-Biology ??

Answer 25

That makes more sense! Wow, thanks @Miracrown How about for Bgl II and Cla I? Would that also mean that there is a cut at 5.5 only?

Answer 26

@Miracrown is on the right direction

you need more double digests to map out Hae II

Answer 27

yeah, I think i figured it those out, but I am confused about it still let me take a picture of the maps i have