Question

Which of the following statements regarding the Sanger sequencing method is accurate? Read carefully!

Answer 1

This method is used to determine the amino acid sequence of a template DNA.


A typical automated sequencing reaction using fluorescence consists of a DNA template, four dNTPs (dATP, dCTP, dGTP, dTTP), a primer, buffer components, DNA polymerase, and four different fluorescently-labeled ddNTPs (ddATP, ddCTP, ddGTP, ddTTP).


DNA sequencing is essentially an in vitro translation reaction.


The Sanger sequencing method works because DNA polymerase can add a nucleotide onto the 3’ end of a ddNTP.

Answer 2

A typical automated sequencing reaction using fluorescence consists of a DNA template, four dNTPs (dATP, dCTP, dGTP, dTTP), a primer, buffer components, DNA polymerase, and four different fluorescently-labeled ddNTPs (ddATP, ddCTP, ddGTP, ddTTP).

Answer 3

This method is used to determine the amino acid sequence of a template DNA.

> sanger sequencing is for DNA not proteins


DNA sequencing is essentially an in vitro translation reaction.

>translation is process of information transfer from a nucleic acid into a protein


The Sanger sequencing method works because DNA polymerase can add a nucleotide onto the 3’ end of a ddNTP.

>ddNTP's dont have a 3' OH, so it stops the chain growth.

Answer 4

Thanks @aaronq I figured it out last night, thanks anyways L:)