Question

1)In the HGD gene shown in IGV-lite, which DNA strand is the template strand?
2)How many amino acids are there in the HGD protein?
3)There is a mutation that changes the C/G base pair at position 38166 to a T/A base pair. What is encoded by the mutated codon?
4)Suppose that a mutation deleted the A/T base pair at location 34670. What would be the last 4 amino acids of the resulting protein? Be sure to use the format Xxx-Xxx-Xxx-Xxx-C where Xxx represent the 3-letter amino acid abbreviations (for example, Arg-Ala-Gly-Ser-C).

Answer 1

U need to show d igv lite

Answer 2

5)There is a mutation that is known to cause alkaptonuria that changes the arginine at position 330 to a serine. This mutation changes one single position from one base to another. Using the DNA sequence encoding amino acid 330 in HGD and a table of the genetic code, determine which base was changed and what the new base is.
What is the number of the base changed by the mutation?
6)What is the new base in the mutant gene? Give the answer in the form of a single letter abbreviation for the base (A, G, C, or T) in the coding (mRNA-like) strand. If there is more than one correct answer give any one of the correct answers.

Answer 3

Without looking at the igv...i can ans a few...to count the no of amino acids...what u have to do is...count the no of nucleotides....den divide dat no by 3. This is because 1 codon which is made up of 3 nucleotides codes for 1 amino acid

Answer 4

i've been trying to post the igv

Answer 5

which ones are the nucleotides?

Answer 6

The template strand is dat strand of the dna which runs 3' to 5'. So d rna formed is from 5' to 3'

Answer 7

Nucleotides are A C G T

Answer 8

in my igv the bottom strand runs fron 3' to 5', does that mean that the bottom strand is the template?

Answer 9

Yes

Answer 10

Ok now....check d 3rd ques...in this ques wat u have to do is...check the codon near the no stated. Check which amino acid it codes for. Then replace g/c with t/a and then check d amino acid this changed codon codes for

Answer 11

are the nucleotides in the protein sequence?

Answer 12

Ok wait wait....lets revise a few things.

Answer 13

DNA is made up of nitrogen bases which are linked together by phosphate group. So a nitrogen base and a phosphate group together make a nucleotide. These nucleotides are adenosine guanosine cytosine and thymidine

Answer 14

When i say only nitrogrn bases they arr adenine guanine cytosine and thymine

Answer 15

ok

Answer 16

Abbreviated as A G C T resp

Answer 17

ok

Answer 18

so i have to count all the "AGCT's" in the protein sequence, right?

Answer 19

DNA is transcribed into RNA. RNA has A C G U. the thymine is replaced by uracil. RNA is single stranded while DNA is double stranded. This single strand of RNA is made up of codons. Codons is nothing but a set of three nucleotides which code for an amino acid. By reading these codons one by one the RNA is translated into protein.

Answer 20

No dear...ACGT's are never found in protein. Its the part of DNA. what all do u know about the basics of dna and rna or overall translation and transription. I am asking this ques so i know from where to start the explanation

Answer 21

i dont really understand it, i just got this assignment. so i dont even know what to do.

Answer 22

Okay...so wat we will do is. Keep this assignment aside for sometime. And lets cover the basics. Shall we?

Answer 23

thing is......its actually due in a few hours

Answer 24

@shrutipande9 are you there?

Answer 25

I sent u a few links...check dat. See if u dnt know d basics we cannot proceed. Bcuz clearly i am not providing u with the answers.

Answer 26

ok, thanks

Answer 27

i have the basics. i went through the links and some others @shrutipande9

Answer 28

in the 3rd question i swapped the c/g for t/a

Answer 29

@beccaboo333 can you help?