Suppose you are a scientist trying to help people who cannot produce an enzyme needed for proper digestion. How could you use genetic engineering techniques to make transformed bacteria that produce the enzyme? MEDALS AND FANS!

Question

anonymous · Answer

Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the same restriction enzyme used to cut out the human gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture containing the antibiotic. These bacteria will make the digestion enzyme.

anonymous · Answer

Can some one change this to where it looks like it wasnt plagirized?

anonymous · Answer

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anonymous · Answer

gtg sorry.

anonymous · Answer

@zepdrix @xXLittleLionXx @Conqueror @vita955 @bibby @nickmifsud1223 @mathmate

anonymous · Answer

its ok

anonymous · Answer

Im lost... sorry :( gtg too im probably not in your grade.. lol

pooja195 · Answer

@PyroYolka I don't think there is another way to write the i did mention I got that from a website,which was similar to openstudy so think of it as the answer to what you asked for.But i think it was the best way to explain it :(

anonymous · Answer

Thanks anyway guys