PLS, HOW DOES A RNA POLYMERASE KNOW WHAT DNA STRAND TO USE AS A GENE TEMPLATE IN THE TRANSCRIPTION UNIT DURING TRANSCRIPTION?
during transcription only one strand of dna is active
How complex are you willing to get? haha What bio are you in?
RNA polymerase binds to a specific promoter - a DNA sequence that provides a recognition and attachment site for RNA polymerase to begin the process of gene transcription. The promoter is upstream from the transcription start site, so the promoter itself does not get transcribed.
obubleoteao is right about this one. there are a number of different good answers depending on what bio you're in. i'll give you the college bio answer in case that's what you're in. if you odn't get what you need from the answer or don't understand then i'll be happy to simplify. RNA polymerase is the protein that binds to sequences of DNA that read TATA or thymine, adenine, thymine, adenine. once it binds to this sequence a few more steps occur before it begins transcribing the downstream gene. because it binds to this sequence which occurs upstream of the gene on the correct strand, the RNA polymerase protein does not frequently read the wrong template.
It is only able to recognize one of the strands during transcription. The RNA pol binds to promoter regions upstream of the gene itself.
obubbleoteao, with all due respect i have no idea what it is ur talking about...by complex you mean what?......another question pls for all, this might be an inane question for some but we know polymerase are proteins but where did the first polymerase come frm if indeed you need polymerase to make protein...and dnt say frm ur parents genes cause you also need a "polymerase" to transcribe the gene for that protein so where did that "polymerase" come frm. or is that one of nature's free gift to you(humans)
Promoters are double-stranded but they are directional. Think about restriction enzyme cut sites, which are palindromic (same read backwards and forwards), well promoters are NOT like that... the RNA polymerase binds to a specific sequence which tells it where to start transcribing the DNA and which direction it should move in.
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