wat is the principle of operation of both a scanning electron microsope and a transmission electron microscope?
Scanning is for viewing things on a bit larger scale (kind of like looking at a whole football field on google maps) while transmission is super close up (like looking at a blade of grass on that field).
An electron microscope fires a stream of electrons at the object, and forms the image by the reflection of those electrons off the target.
tanxs
No probs
SEM involves the scatter of electrons from am object coated with a dense (probably metallic) "coat". it deals with the surface of an object. imagine pollen surface or cool creepy 3d bacteria in TEM. the beam is shot through an ultra thin slice of specimen. the result is 2d. the resolution is mainly at the organelle level.
Very high resolution, Samples must be fixed (Vacuum), contrast requires heavy metal staining (artifacts + loss of resolution). Those are the similarities which I think is what your asking..
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