Question

is anyone here who works in lab or research centre as a student i just want some help y dont u guys prepare a stain pic in which we can see P S1 and PS2 chlorophyll exactly where it is located in leaf ...
plz think abut this.. this will be grt help to us.. thanQ :)

Answer 1

superb idea. Hope this helps me when i study biology in detail.

Answer 2

i work in a research lab, but all i deal with is neurochemistry and behavior. if i was in botany i would surely help. :p

Answer 3

neurochemistry grt... awesome ...
can u give me the video of actuall working going within eye that how the impules tranfer from horizontal cell etc. plz thanQ

Answer 4

i can see what i can get for ya. :)

Answer 5

thanQ... so much...

Answer 6

Video of impulse transfer? Will be cool stuff if anybody can get that; given that impulse travells faster than most lab camera's. Somebody will have to understand the process and animate it for you I guess.

I doubt that it is possible.

Answer 7

Supporting Information
Cardol et al. 10.1073/pnas.0908111106
SI Methods
Isolation of the dum22stt7–9 Strain. The meiotic progeny of the
cross was obtained after dissecting tetrads or octads. The first
analysis was for the presence of the mitochondrial dum22
mutation that prevents growth in the dark caused by the inability
to use acetate as an exogenous source of reduced carbon through
respiration (dark phenotype). About 80% of the progeny (15 of
the 19 clones obtained from 9 germinated zygotes) showed a
dark phenotype (Fig. S1), in good agreement with the preferential
transmission of the mitochondrial genome by the mt
parental strain (1). Respiratory deficiency was confirmed further
in the 15 dark strains by performing an in vivo 2,3,5-
triphenyltetrazolium chloride (TTC) staining test (1). When
placed in anaerobic and dark conditions for few hours, wild-type
colonies reduce TTC to red formazan and stain purple, whereas
respiratory mutant colonies remain green (Fig. S1 and Table S1).
The presence of the nuclear stt7–9 mutation then was assessed
based on the loss of ST among the dark progeny. To this end,
we measured the ratio of PSI over PSII fluorescence emission at
77 K, at which the light emission by each photosystem can be
distinguished. Under conditions in which the PQ pool is reduced,
thus promoting state 2 (i.e., in the dark for the dum22 mutants
or in the dark under anaerobiosis for the wild-type cells), most
of the antenna proteins are connected to PSI, resulting in a
stimulation of PSI fluorescence emission at the expense of that
of PSII. The opposite situation prevails in the state 1 condition,
here achieved by illumination in the presence of DCMU to
oxidize the PQ pool (2). In agreement with previous reports, a
switch from state 2 to state 1 conditions was accompanied by a
decrease in the PSI/PSII fluorescence ratio in the wild-type or
dum22 mutant cells, but no changes were seen in stt7–9 cells
(Table S1and Fig. S1), which are constitutively locked in state 1
because of the absence of the Stt7 kinase. Based on their
constant fluorescence ratio, 7 dum22stt7–9 clones were isolated
among the 15 isolates carrying the dark phenotype, thus
confirming the Mendelian inheritance of the stt7–9 nuclear
mutation. We further analyzed 2 of these clones (A1, J1) for their
photosynthetic features.

Answer 8

^^ What is that Barry?