Question

Hey! I'm quite confused about the recombinant DNA thingy...:(
Here my question! For example, after a black gene allele is extracted from a plant, which plasmid should combine with this gene before put into a particular bacteria? How do i know which plasmid is suitable for this gene??

Answer 1

As long as the plasmid is cleaved having a satisfying sequence of sticky ends.
And just as related, the same restriction enzymes are used to digest the plasmid and the gene of interest. THe kind of bp sequence must manage to associate with the gene the right base pairing.
Then it is in point and in fact going to "retain" the gene, to produce a recombinant DNA Sequence in the plasmid.

Answer 2

So does it means no matter what type of bacteria used, as long as the sticky ends are the same as the gene and they are cut by the same restriction enzyme?

Answer 3

selection of plasmid is based on the size of the gene of interest which is going to be ligated.
for example in a normal plasmid we can integrate our gene of interest whose size is 5Kb,
cosmid can carry 25Kb sized gene.

and we should check for the presence of the restriction site in the plasmid which was used to isolate the gene of interest.