Question

Why is a marker used when running the fragments through the gel?

Answer 1

We use a marker (fragments with known lengths of polymers) so we can compare with our samples.

Answer 2

like how does it compair?

Answer 3

I have a old pic where i made a gelelektrofrese with a protein or DNA i think... can use that as an example?

Answer 4

sure

Answer 5

wait what?

Answer 6

?

Answer 7

1 and 12 are all known numbers of bps. 2-10 is unknown. We like to how big the sample of DNA was in 2-10 and we do so by comparing the DNA samples with the marker, for an exaplle take a look at nr. 2. we can see that it almost lie just outside the marker and that marker point that it lies just outside is about 450 bps... so we can say that it most be a bit less than 450 bps on the DNA sample 2.

Answer 8

PCR and that kind of ways of analyzing DNA or proteins is normely just a minor part of an experiment, but this is how it is done.

Answer 9

sooo markers show...i must sound really stupid...um in two sentnces please summarize that in third grader language?

Answer 10

??

Answer 11

The markers are of a specific size (in numbers of base pairs). By comparing the rates of migration of the various markers with the rates of migration of the gene/genes you are separating, you can get an estimate of the size of the fragments you are interested in. They also let you know if you need more or less separation. If the smaller fragments run off of the gel, you'll know that you need to run if for a shorter time or with a smaller voltage.

Answer 12

you got that from yahoo answers :/

Answer 13

yep, cause i dont know the answer, lol didnt know if you read this or not,but i guess so ahahaha

Answer 14

http://wiki.answers.com/Q/Why_is_a_marker_used_when_running_the_fragments_through_the_gel its the same as your answer

Answer 15

oh dear i just wish it could be explained in a very kindergarnerishway lol

Answer 16

is this for homework or just to know? I go to connections academy

Answer 17

i do keystone onloine academy im doing a lab if i get an answer i will NOT copy and paste it into my answer i just need to understand it so i can write it in my own words

Answer 18

help?

Answer 19

i dont know the answer :/ i would help if i knew it...

Answer 20

i have been trying to understand this one question for over an hour..i am really behind i need help

Answer 21

two words: the marker is like a ruler, by useing the marker as a ruler we can say how "long" the DNA is.

Answer 22

oh so the marker tell you how long the DNA is

Answer 23

Yes! ;D

Answer 24

so it says how big it is?

Answer 25

Yea and normely we use the unit cm or m... but with DNA we use the unit base pairs.. the number of bases.

Answer 26

fx. TGGGTGTTTGT would be 11 base pairs (bp)

Answer 27

ooooh so the marker measures the dna fragments with by basesinstead of like an inch or centemetrer?

Answer 28

100% true yes

Answer 29

THANK YOU!!!!!!!! i would fan you again if i could ...but i cant so you gat a medal:) XD

Answer 30

Your so much welcome... perhaps i should start using some analogies some more.. ^^

Answer 31

yeah that might help:)