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OpenStudy (anonymous):

What is a restriction map?

OpenStudy (anonymous):

A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes

OpenStudy (anonymous):

what is a restriction site?

OpenStudy (frostbite):

lol?

OpenStudy (anonymous):

whats so funny?

OpenStudy (anonymous):

like where i found it??

OpenStudy (frostbite):

Well tammy said it quite clear. never the less i know you so good now that i guess you want it simplifyed? ^^

OpenStudy (anonymous):

haha of course:)

OpenStudy (anonymous):

and no i do not have a low IQ i just like taking things in their simplist forms

OpenStudy (frostbite):

EmmaH one thing: That thought never orcure to me, the only reason i can this is becuase i ones was in your positon and asked for answers - So i don't judge. anyway back to the case.

OpenStudy (anonymous):

thanks:)

OpenStudy (frostbite):

See attachment: A restriction map tell you where on DNA string where a specific restriction enzyme is going to work: A analogy for this would be a real map: imagen you have a lot of cities with specific names (they are enzymes) and the whole map discribe how the cities are placed acording to each other.

OpenStudy (frostbite):

The restriction map i used as example is the plasmid pGLO

OpenStudy (frostbite):

Remember that plasmids are DNA circles that is why it is a circle on the figure in the attachment.

OpenStudy (anonymous):

ok i get the fact that a restriction map shows where ba restriction enzyme is but..what is a restriction enzyme?

OpenStudy (frostbite):

A restriction enzyme is a enzyme that is able to cut the DNA into smaller strings every time there is a specfic sequence of bases.

OpenStudy (anonymous):

oh ok so the restricted enzymes are like the cutting toold for when you want to splice dna and the restiction nmap nshows where restiction dna is right?

OpenStudy (frostbite):

The most common thing is that it is a palindromic sequence

OpenStudy (frostbite):

yea

OpenStudy (anonymous):

palindromic sequenence?

OpenStudy (frostbite):

Emm... palindromic comes from the Greek word palindromos and means 'coming again, which again goes the same way'. (symmetric)

OpenStudy (anonymous):

alright so i got it earlier when i said.....the restricted enzymes are like the cutting toold for when you want to splice dna and the restiction nmap nshows where restiction dna is right?

OpenStudy (frostbite):

Well: more to say that the restiction map show where restiction enzyme works on the DNA... kinda like a blueprint

OpenStudy (frostbite):

Else you are right.

OpenStudy (anonymous):

ooooooh ok thanks! got it

OpenStudy (frostbite):

Last thing: this is just to show the concept of a palindromic sequenence (see attachment)

OpenStudy (anonymous):

thanks:)

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