Question

What percentage gel should be used if you want to separate DNA fragments that are 25,000 bp , 21,000 bp, 10,000 bp and 6,000 bp?

Answer 1

hvent learnd it :(

Answer 2

Sorry, I haven't learnt this either.

Answer 3

Hmmm what kind of gel? almost guessing agarose?

Answer 4

If we don't have this factor it can be hard to know becuase the driving force of the molecule or DNA is given by:
\[f=6 \pi \eta r\]
Here f is the driving force, η is the viscosity of the medium and r is the radius of a sphere.

Answer 5

But if using a agarose gel I would say about 0,5 % agarose, that should cover 1k to 30k.

Answer 6

oh wow, thank you! thank you so much!!!!

Answer 7

and standard plate btw. ;)

Answer 8

@Frostbite dude continue helping her :D please! shes in ap bio XD

Answer 9

Not sure I follow?

Answer 10

Oh. She's Advanced Placement Biology and she needs help haha.

Answer 11

I'm in ap bio and i don't get this lab report at all haha

Answer 12

Well this is college bio, so I wonder if you don't have any tables to look in, I only found it, becuase I had a old table from a prof of mine. The eqautions however is stright on ;)

Answer 13

ARTI ASK THE NEXT QUESTION CHILD

Answer 14

@Frostbite could you help me with some other questions? :)

Answer 15

Maybe, I'll give it a shot.

Answer 16

you perform a restiction digest that should yield 1400 bp, 1080 bp, and 400 bp fragments. You check the restiction digest by running it on a gel that is 1.5 %, the correct percentage. but you're in a rush and only partially run the gel you also forget to load the marker

Answer 17

when you stain the gel you only see three bands in the lane you loaded. The second band from the well is more intense than thr others and looks like a doublet. which 2 fragements make up the double? why? what could you do to further separate the fragemnts to verify this?

Answer 18

Hmmm, not quite sure about this sorry.

Answer 19

oh, okay thank you though! how about this one ----> if you want to run gel electrophoresis at 100 v for two hours instead of one (which is how long it should take) what should you do?

Answer 20

Hmmm an increase in running time will allow the bands to separate even more... wich you ofcuase need to take into account if you make a aproximation. Beside from that, I most again say I'm not sure.

Answer 21

Might also need to adjust some equations if you have some formular that have bps as a function of length of bands travel.

Answer 22

i was thinking that you could rin it at half the voltage (50 v)

Answer 23

Oh, then i misunderstod well depends if voltage and time is porportional ;)

Answer 24

haha i don't really know. I'm just going to out that down and hope for the best!