Can anyone explain to me how to prepare 500 ml of buffer containing 3%(w/v) PVP by using PVP powder and Distilled water?I only want the instruction on how to do it...what does (w/v) mean and etc.

Question

koikkara · Answer

This common usage of % to mean wt/v in biology.

anonymous · Answer

Then do you know how it can be answered?

koikkara · Answer

@terenzreignz:  @opcode @AriPotta:....



well, thinking.....

anonymous · Answer

ok

unklerhaukus · Answer

percentage weight of solute per volume of solution

if %(w/v)=x, then there are  x grams of solute for every 100 mls of solution

anonymous · Answer

Ok thanks a lot :)

koikkara · Answer

http://histemb.medicine.ankara.edu.tr/Cryopreservation%20and%20Freeze-Drying%20Protocols.pdf may help u...

anonymous · Answer

Thanks a lot :)

unklerhaukus · Answer

note that the amount of water in the solution is not necessarily 100 ml

anonymous · Answer

Then the 100 ml is the normal one,right?

unklerhaukus · Answer

100 mls is the unit of the solution

anonymous · Answer

Then at first,I should weight 3 grams of PVP and then add water proportional to have 500 ml,am I right?

unklerhaukus · Answer

well that would make a    0.6%(w/v) solution

unklerhaukus · Answer

you need more powder

anonymous · Answer

How do you calculate?Any formula?Sorry for a lot questions.

unklerhaukus · Answer

$$3\%(w/v)=\frac{3[\text g]}{100[\text{mls}]}$$

$$500[\text{mls}]\cdot3\%(w/v)=500[\text{mls}]\cdot\frac{3[\text g]}{100[\text{mls}]}=...[\text g]$$

anonymous · Answer

Thanks...really helped ^.^