Question

What is the molar extinction coefficient of the enzyme endo-polygalacturonase from Aspergillus niger? (EC=3.2.1.15)

Answer 1

Anyone knows where i can find this?

Answer 2

Have you tried reaxys?

Answer 3

Never heard of reaxyx.. it seems I have to log in there?

Answer 4

can't find it with uniprot either

Answer 5

I think I found what you're looking for through uniprot here: http://www.uniprot.org/uniprot/P26214

The amino acid sequence of the protein would be:
MHSFASLLAY GLVAGATFAS ASPIEARDSC TFTTAAAAKA GKAKCSTITL NNIEVPAGTT LDLTGLTSGT KVIFEGTTTF QYEEWAGPLI SMSGEHITVT GASGHLINCD GARWWDGKGT SGKKKPKFFY AHGLDSSSIT GLNIKNTPLM AFSVQANDIT FTDVTINNAD GDTQGGHNTD AFDVGNSVGV NIIKPWVHNQ DDCLAVNSGE NIWFTGGTCI GGHGLSIGSV GDRSNNVVKN VTIEHSTVSN SENAVRIKTI SGATGSVSEI TYSNIVMSGI SDYGVVIQQD YEDGKPTGKP TNGVTIQDVK LESVTGSVDS GATEIYLLCG SGSCSDWTWD DVKVTGGKKS TACKNFPSVA

Feeding that protein sequence into ExPASy results in the following extinction coefficient:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 49430
Abs 0.1% (=1 g/l) 1.318, assuming all pairs of Cys residues form cystines

Answer 6

Hm actually i need the molar extinction coefficient for endopolygalacturonase I (pga1)
That's P26213 on uniprot. How did you find that information? You must have clicked somewhere to get that because it's not on the main page of P26214..

Answer 7

@thomaster

I followed links from PDB crystal structures for endopolygalacturonase I and II.

http://www.rcsb.org/pdb/results/results.do?qrid=30E75512&tabtoshow=Current

Answer 8

Still can't find it... \(\Huge\ddot\frown\)

Answer 9

@nincompoop Thanks!!