A scientist modified the structure of a promoter region of a gene. What could be its effect on transcription and translation? A. RNA polymerase no longer binds to the DNA, so DNA will be directly translated into protein. B. RNA polymerase will now bind to the coding region of the gene and carry out transcription. C. RNA polymerase may not be able to bind to the DNA, so transcription will not occur
The key to this question is knowing WHAT the promoter regions task is on a gene and how it regulates transcription.
CONCEPT: ACTIVATION OF GENE EXPRESSION I think every person has a good hands on the question, but no one really directly answered your question based on the choices given. The foundation required here is the role of the promoter. IT selectively turns on the gene encoding enzyme that organisms need to make their own supply of the 3 of 8 essential amino acids leucine, isoleucine and valine - the ALS (acetolactase synthase) gene. When this gene is TURNED ON, TRANSCRIPTION CAN TAKE PLACE. Thus, modifying the promoter can mean two things: INHIBITING OR ENHANCING. To Inhibit ALS would mean TRANSCRIPTION CANNOT TAKE PLACE; while enhancing ALS would just mean an increased in transcription activity. TIP: transcription is the keyword, pal.
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