Question

Given 20 mM HEPES buffer (pH 7.0) with 100 mM NaCl Show how you would make 500 ml of this buffer. I will allow you to use no pH meter, but pretend you were given the following:
1. HEPES dry powder: (CAS Number 7365-45-9, Empirical Formula C8H18N2O4S, Molecular Weight 238.30; pKa 7.5)
HINT: What form of the compound am I giving you?
2. 1.0 M solutions of NaOH and HCl
HINT: You will have to decide to use one of these or both of these, then calculate how much to use.
3. 5.0 M NaCl
please help

Answer 1

how will i start this

Answer 2

You want to make a buffer that is +1 and -1 from the pka of the HEPES, which will be 6.5 to 8.5. This is a the buffer region.

Not sure what the NaCl is for.

Answer 3

what do you mean please

Answer 4

i dont know!!!!!

Answer 5

do you know how to start at a;ll