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Chemistry
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Ah buffers... So, i need to make 1 L of a 0.06 M buffer of Histidine at pH=5.5. Im given 1.0 M Histidine (full deprotonated), 1.0 M HCl and 1.0 M NaOH. So, right away i added 0.12 moles of HCl to bring the histidine to the right form. So to start i used henderson hasselbach: 5.5 = 6 + log\(\dfrac{A-}{HA}\) \(\dfrac{A-}{HA}\)=0.316 %[HA]=0.76 %[A-]=0.24 \(n_{HA}\)=0.76*0.06M*1L=0.0456 moles \(n_{A^-}\)=0.24*0.06M*1L=0.0144 moles SO i need to put in: \(n_{HA}\)=0.06 moles and \(n_{NaOH}\)=0.0144 moles \(V_{HA}=\dfrac{0.06moles}{1M}\) \(V_{NaOH}=\dfrac{0.0144 moles}{1M}\)
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